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Fig. 4 | Virology Journal

Fig. 4

From: Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman

Fig. 4

Southern blot analysis of DNA extracted from agroinoculated N. tabacum plants. Blots were probed for the presence of ChiLCV (a) and ToLCB (b). The samples run on the gels were extracted from Nicotiana glutinosa, N. benthamiana, and N. tabacum plants (as indicated) inoculated with only ChiLCV (lane 1 panel A) or co-inoculated with ChiLCV and ToLCB (lane 2 in panels A and B). In addition DNA extracted from one of the field-infected tobacco plants (F) and from a healthy, non-inoculated tobacco plant (H) was included as controls. In each case approx. 10 μg of DNA was loaded. For each blot a photograph of the ethidium bromide-stained genomic DNA band on the gel is shown to confirm equal loading. The positions of viral/betasatellite single-stranded (ss), supercoiled (sc) and open-circular DNA forms are indicated

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