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Table 5 Evaluation of the effect of delay between diluting virus and adding cells on MPBT assay results (log10CCID50/ml)

From: Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Sabin Strain

Delay in minutes

Mean

SD

RSD %

15

30

45

60

1

8.75

8.88

9.13

8.88

8.91

0.16

37.86

2

8.75

8.63

8.63

8.50

8.63

0.10

23.84

3

9.25

8.88

9.13

9.00

9.07

0.16

38.15

  1. SD Standard deviation of log10 titers, RSD Relative standard deviation
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Virology Journal

ISSN: 1743-422X