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Table 4 Evaluation of the effect of cell numbers per well variation on the MPBT assay results (log10CCID50/ml)

From: Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Sabin Strain

Cell numbers per well

Mean

SD

RSD %

1 104

2 104

4 104

1

8.5

9.12

8.62

8.75

0.33

87.98

2

8.5

8.38

8.5

8.46

0.07

16.05

3

8.62

8.88

8.88

8.79

0.15

35.62

  1. SD Standard deviation of log10 titers, RSD Relative standard deviation