Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Table 2 Determination of the low limit of titration (LOT) of OPV viruses by MPBT assay and its comparison with LOT of CCID50 assay

Assay	Sabin strain combinations	Titer of the analyzed viruses (CCID₅₀/ml)
Assay	Sabin strain combinations	100	50	25	10	5	1	0.1
MPBT	1	125.89	81.28	35.48	31.62	NT	19.95	UD
CCID50	1	158.49	87.10	50.12	22.39	15.85	17.78	UD
MPBT	2	123.03	51.29	28.18	22.39	17.78	UD	UD
CCID50	2	95.50	36.31	35.48	19.95	17.78	UD	UD
MPBT	3	104.70	56.23	30.90	15.85	19.95	UD	UD
CCID50	3	95.50	51.30	33.88	16.98	15.85	UD	UD
MPBT	1 2 3	104.71	39.81	36.31	17.78	15.85	UD	UD
		74.13	74.13	21.88	19.95	17.78	UD	UD
		56.23	28.18	21.88	19.95	15.85	UD	UD

ISSN: 1743-422X