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Table 3 Intra- and Inter-assay coefficients of variation for duplex TaqMan Real-time RT-PCR

From: Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses

Dilution of RNA

n

Intra-assay(%)

 

Inter-assay(%)

 
  

H5

N6

H5

N6

10−1

4

1.12%

1.83%

1.41%

1.68%

10−3

4

1.50%

1.71%

1.25%

2.29%

10−5

4

1.42%

1.31%

1.11%

1.13%