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Table 3 Intra- and Inter-assay coefficients of variation for duplex TaqMan Real-time RT-PCR

From: Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses

Dilution of RNA n Intra-assay(%)   Inter-assay(%)  
   H5 N6 H5 N6
10−1 4 1.12% 1.83% 1.41% 1.68%
10−3 4 1.50% 1.71% 1.25% 2.29%
10−5 4 1.42% 1.31% 1.11% 1.13%