Fig. 2
The hemagglutinin (HA) and Neuraminidase (NA) plasmids of a highly pathogenic H5N6 virus (DK/GX/1/18) were 10-fold serially diluted with ddH2O, and 10−1–10−10 dilutions were used to evaluate the TaqMan real-time RT-PCR method. Amplification curves are shown in the left panel in descending order of the template concentration with the highest concentration (10− 1) on the left. a. The standard curve is shown in the right panel. The pHA plasmid displayed a slope of −3.442, an intercept of 47.123, a correlation coefficient of 0.996, and an amplification efficiency of 95.218% b. The pNA plasmid dilutions from 10− 3 to 10−7 displayed a slope of − 3.443, an intercept of 40.804, a correlation coefficient of 0.999, and an amplification efficiency of 95.192% (c)