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Fig. 3 | Virology Journal

Fig. 3

From: Characterization of Cronartium ribicola dsRNAs reveals novel members of the family Totiviridae and viral association with fungal virulence

Fig. 3

Viral RNA abundance of Cronartium ribicola totiviruses (CrTV1 to CrTV5) as measured by qRT-PCR analysis. Total RNA levels were normalized across 33 tested fungal samples using C. ribicola α tubulin transcript as the calibrator. Relative RNA levels were calculated using the 2−ΔΔCt algorithm. Means for relative RNA levels of each virus genome were analyzed in three comparisons: spores vs. in planta mycelium growth in infected pine tissues; in planta mycelium growth in three host plants: WWP-western white pine (Pinus monticola), WBP-whitebark pine (P. albicaulis), and LP-limber pine (P. flexilis); and C. ribicola pathotypes vcr2 vs. avcr2. Bars show average RNA levels of each virus with standard error of the mean (SEM). Different letters on the tops of the bars indicate significant difference (t-test, P < 0.05) among three host plants. One, two, and three stars (*) indicate P < 0.05, P < 0.01, and P < 0.001, respectively, in paired comparisons

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