Table 2 Primers used for construction of D51 infectious clone
From: A novel method to rescue and culture duck Astrovirus type 1 in vitro
Primer | Sequences(5′-3′) |
|---|---|
HeadRiboF1 | TTGGCGCGCCACATCATCTGATGAGTCCGTGAGGACGAAACGGTACCCG (Asc I) |
HeadRiboF2 | CGTGAGGACGAAACGGTACCCGGTACCGTCATCCGAAGTGGGCGAGTCGGGGCCATGGC |
F1-R | CCAGGTGTCAACAATCATGC |
Long-7R | CCAGAACATTGTCTTTATTCCCTGTAATCTGTCCAAGTGTGACCACTGTTGTC |
Long-8F | AGGTGCAGGGTCCCCCTGTCAATGATAAAATGACAACAGTGGTCACACTTGGA |
aR | CGCGGATCCTTTGGATACGCTGGTGTTG (BamH I) |
bF | CAGAGAATGCTTGATGAAGGC |
bR | CGCGGATCCGTTGAAAACTGCCCTGAAGG (BamH I) |
cF | CTTGGACTGTGGAAGCATATACC |
cR | CGCGGATCCTCATGTGTGGTGTATACTGTGATG (BamH I) |
dF | GACAACAGTGGTCACACTTGGA |
F12-F | ACAGCTGCACTTTCTCATGC |
HpaR1 | CCGCTCGAGGTCCCATTCGCCATTACCGAGGGGACGGTCCCCTCGGAATGTTGCCCAGCCG (Xho I) |
HpaR2 | CGGAATGTTGCCCAGCCGGCGCCAGCGAGGAGGCTGGGACCATGCCGGCCTTTT |
HpaR3 | GGACCATGCCGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGCCAATTGAA |
Marker1 | AGCTGGATGTCTGTATTTCGGTTCAACAGCCTCCAGATCTCTCAACAGCGCAC (Bgl II) |
Marker2 | GCTGTTGTGGTGAAGATCAGTGGTGCGCTGTTGAGAGATCTGGAGGCTGTTGAACC (Bgl II) |