From: A novel method to rescue and culture duck Astrovirus type 1 in vitro
Primer | Sequence | Localization (in the polyprotein gene) | Fragment length (bp) | |
---|---|---|---|---|
1F | CCGAA(G/A)TGGGCGAGTC | 1–918 | 919 | |
1R | CCAGGTGTCAACAATCATGC | |||
2F | GATGTTTATGGTGAGTTGTACAC(A/T)G | 806–1786 | 972 | |
2R | GCTAGATGGTATTATGCCTCTTG | |||
3F | TTGCAGCTCACTCTGGCATA | 1567–2231 | 666 | |
3R | TGATGTCACTCCCTGTGAA(G/C)C | |||
4F | CTCGCGAAAGAAGTTAAGAGTG | 2021–2989 | 971 | |
4R | CCTTCTTCATCGATGTCATTATCC | |||
5F | CAGAGAATGCTTGATGAAGG(C/T) | 2879–4177 | 1297 | |
5R | TT(T/A)GGATACGCTGGTGTTGA | |||
6F | CTTGGACTGTGGAAGCATATACC | 3977–4740 | 763 | |
6R | GTTGAAAACTGCCCTGAAGG | |||
7F | GTTACCAACTGGAGAAGTCTGTCA | 4680–5582 | 903 | |
7R | TCCAAGTGTGACCACTGTTGTC | |||
8F | GACAACAGTGGTCACACTTGGA | 5561–6619 | 1082 | |
8R | CCATGTGTGGTGTATATTGTGAT(G/A) | |||
9F | GAGGTGTTCTGGGCAGTTTCAA | 6291–7403 | 1113 | |
9R | CTCCATCATCATCCTCTCACACTG | |||
10F | AGTGTGAGAGGATGATGATGGA | 7381–7620 | 240 | |
10R | TGTGACCCACATGGTGATTTC | |||
3′ Race | P1 | AGTGTGAGAGGATGATGATGGA | 7381–7751 | 371 |
Race1 | GACTCGAGTCGACATCGA(T)18 | |||
P2 | ACAGCTGCACTTTCTCATGC | 7516–7751 | 236 | |
Race2 | GACTCGAGTCGACATCGA |