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Table 1 List of primers used in this study

From: Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems

Primer

Sequence 5′ → 3´

Description

Kan control

F: CGTACTCCTGATGATGCATG

R: ATTCGTGATTGCGCCTGAGC

For control PCR/sequencing after first recombination into MVA-BAC

MVA DelIII

F: GATGAGTGTAGATGCTGTTATTTTG

R: GCAGCTAAAAGAATAATGGAATTG

To check the presence of WT and gene insertion after plaque picking

MVA DelVI

F: CTCCGCATCTAGTTGATATTCCAACCTCTT

R: CCTGGACATTTAGTTTGAGTGTTCCTGAAT

For first homologous recombination into the MVA-BAC by En-passant

pEPH5-GII4

F: CATAAATAAGGTTGACTCTAGAGCCACCATGAAGATGGCCTC

R: ACGTAGAGCTCTTAAGGAATTCTTATACGGCTCGTCTTCTACCT

For cloning of NoV GII.4 into pEP-MVAdVI-PH5 plasmid

pIIIH5-GII.4

F: TAAGGTTGACTCTAGAGCTAGCGCCACCATGAAGATGGCCTC

R: CGGCCGCGTTTAAACCTCGAGTTATACGGCTCGTCTTCTACCT

For cloning of NoV GII.4 into the pIIIH5 shuttle vector

RFV

F: AAAGATGCGTACATTGGACCC

R: GTTCGAGACTAGAAAAGCGCC

To check the presence of helper virus RFV in the transfect cells with recMVA-BAC