Primer | Sequence 5′ → 3´ | Description |
---|---|---|
Kan control | F: CGTACTCCTGATGATGCATG R: ATTCGTGATTGCGCCTGAGC | For control PCR/sequencing after first recombination into MVA-BAC |
MVA DelIII | F: GATGAGTGTAGATGCTGTTATTTTG R: GCAGCTAAAAGAATAATGGAATTG | To check the presence of WT and gene insertion after plaque picking |
MVA DelVI | F: CTCCGCATCTAGTTGATATTCCAACCTCTT R: CCTGGACATTTAGTTTGAGTGTTCCTGAAT | For first homologous recombination into the MVA-BAC by En-passant |
pEPH5-GII4 | F: CATAAATAAGGTTGACTCTAGAGCCACCATGAAGATGGCCTC R: ACGTAGAGCTCTTAAGGAATTCTTATACGGCTCGTCTTCTACCT | For cloning of NoV GII.4 into pEP-MVAdVI-PH5 plasmid |
pIIIH5-GII.4 | F: TAAGGTTGACTCTAGAGCTAGCGCCACCATGAAGATGGCCTC R: CGGCCGCGTTTAAACCTCGAGTTATACGGCTCGTCTTCTACCT | For cloning of NoV GII.4 into the pIIIH5 shuttle vector |
RFV | F: AAAGATGCGTACATTGGACCC R: GTTCGAGACTAGAAAAGCGCC | To check the presence of helper virus RFV in the transfect cells with recMVA-BAC |