Skip to main content

Table 1 List of primers used in this study

From: Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems

Primer Sequence 5′ → 3´ Description
Kan control F: CGTACTCCTGATGATGCATG
R: ATTCGTGATTGCGCCTGAGC
For control PCR/sequencing after first recombination into MVA-BAC
MVA DelIII F: GATGAGTGTAGATGCTGTTATTTTG
R: GCAGCTAAAAGAATAATGGAATTG
To check the presence of WT and gene insertion after plaque picking
MVA DelVI F: CTCCGCATCTAGTTGATATTCCAACCTCTT
R: CCTGGACATTTAGTTTGAGTGTTCCTGAAT
For first homologous recombination into the MVA-BAC by En-passant
pEPH5-GII4 F: CATAAATAAGGTTGACTCTAGAGCCACCATGAAGATGGCCTC
R: ACGTAGAGCTCTTAAGGAATTCTTATACGGCTCGTCTTCTACCT
For cloning of NoV GII.4 into pEP-MVAdVI-PH5 plasmid
pIIIH5-GII.4 F: TAAGGTTGACTCTAGAGCTAGCGCCACCATGAAGATGGCCTC
R: CGGCCGCGTTTAAACCTCGAGTTATACGGCTCGTCTTCTACCT
For cloning of NoV GII.4 into the pIIIH5 shuttle vector
RFV F: AAAGATGCGTACATTGGACCC
R: GTTCGAGACTAGAAAAGCGCC
To check the presence of helper virus RFV in the transfect cells with recMVA-BAC