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Fig. 7 | Virology Journal

Fig. 7

From: Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems

Fig. 7

Biochemical characterization of recMVAs. a Binding assay: Control cells are depicted on the left side. Virus binding to DF-1 cells was visualized by staining with anti-Vaccinia virus antibody. Top: recMVAs derived by the marker-based method; Bottom: BAC-recMVAs. b Electron microscopy with negative staining. Scale: 300 nm. c Expression of NoV VP1 in DF-1 cells infected with recMVAs. Immunoblotting was carried out using human serum from GII-infected patients as primary- and anti-human IgG as secondary antibody. VP1 protein (58 kDa) was detected in both recMVAs. Non-infected- and WT-MVA-infected cells were used as negative controls. Recombinantly expressed GST-tagged GII.4VP1 was used as positive control. d Schematic workflow for recMVA generation using traditional approach (left) and MVA-BAC system (right)

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