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Fig. 2 | Virology Journal

Fig. 2

From: Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems

Fig. 2

Schematic overview of MVA-BAC technique for generation of recMVA. a In E. coli GS1783, following induction at 42 °C, the gene of interest together with I-SceI-Kan fragment is inserted into the DelVI site of the MVA-BAC by homologous recombination. Co-integrated Kan cassette (aphAI) is removed by induction of I-SceI production by arabinose followed by second Red recombination b In DF-1 cells, recMVAs are rescued in present of a helper virus and the BAC backbone including GFP cassette is removed spontaneously upon passaging

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