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Fig. 7 | Virology Journal

Fig. 7

From: Identification of virion-associated transcriptional transactivator (VATT) of SGIV ICP46 promoter and their binding site on promoter

Fig. 7

Effect on SGIV ICP46 promoter activity by transient expression of four VATTs. (a) Expression of four VATTs in GS cells verified by RT-PCR after transfected with pcDNA-012, pcDNA-039, pcDNA-057 and pcDNA-072 at 24 h post tansfection . lanes M, 10 kb DNA ladder; Lanes C, negtive control. (b) The luciferase reporter assays after co-transfection with pcDNA-012, pcDNA-039, pcDNA-057, pcDNA-072 alone or 4 plasmids combined. At 24 h post transfection, cells were infected with SGIV and harvested at 6 h p.i.. The co-transfected cells without SGIV infection were harvested at the same time for luciferase reporter assays. Firefly luciferase activities were normalized based on the activity of Renilla luciferase. The mean Fluc/Rluc intensity of negative control was considered as 1

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