Fig. 6From: Identification of virion-associated transcriptional transactivator (VATT) of SGIV ICP46 promoter and their binding site on promoterSDS-PAGE of DNA pull-down assays. DNA pull-down was performed with the biotin-labled probe X and the protein extraction from AraC+SGIV (lane 3), CHX + SGIV (lane 4), SGIV+GS (lane 5), GS cells (lane 6) GS, and purified SGIV virion (lane 7), respectively. Streptavidin-agarose bead (lane 1) was used as negative control and lane 2 is protein maker. Band A and B show the two DNA-binding proteins obtained in sample SGIV by DNA pull-down. Arrows show the protein bands choosed for NanoLC-ESI-MS/MS analysisBack to article page