Skip to main content
Fig. 5 | Virology Journal

Fig. 5

From: Identification of virion-associated transcriptional transactivator (VATT) of SGIV ICP46 promoter and their binding site on promoter

Fig. 5

Electrophoretic mobility shift assay. The whole protein extractions from AraC-pretreated and SGIV-infected GS cells (AraC+SGIV, lane 3), CHX-pretreated and SGIV-infected GS cells (CHX + SGIV, lane 4), SGIV-infected GS cells (SGIV+GS, lane 5), GS cells (GS, lane 6) and purified SGIV virion (SGIV, lane 7) were obtained for EMSA analysis with biotin-labeled probe X that contains the sequence of SGIV ICP46 promoter. Incubated with biotin-labeled probe NFκB, the nuclear extracts with activated NFκB was used as positive control (lane 1) and nuclear extracts without activated NFκB (lane 2) was used as negative control. Arrow show the DNA binding proteins; NSB, non-specific binding; P, free biotin labeled probe

Back to article page

Virology Journal

ISSN: 1743-422X