Fig. 4

Regulation of the SGIV ICP46 promoter. a The transcription of SGIV ICP46 requires SGIV infection and is activate by heat-labile virion-associated transcriptional transactivator (VATT). GS cells were transfected with P-22, which containing the core region of SGIV ICP46 promoter, and then the transfected cells were either mock infected or infected with infectious SGIV, heat-inactivated SGIV and UV-inactivated SGIV, respectively. b CHX and Ara has no significant effect on the activity of SGIV ICP46 promoter. GS cells were transfected with P-22 and then the transfected cells were pretreated with cycloheximide (CHX) or cytosine arabinofuranoside (AraC) for 1 h before and throughout the SGIV infection. CHX or AraC-pretreated cells were infected with SGIV, then harvested at 6 and 48 h p.i., respectively. The P-22 transfected GS cells infected with SGIV were collected at the same time points and served as control