Table 1 The specific primers for Nsp3
Gene name | Primer name | Primer sequence (5′-3′) | Position (bp) |
|---|---|---|---|
NSP3–1 | Nsp3(1-418aa)-F | ACGCGTCGACCGGTGACAAAACTGTCTCATTTTCAG | 1–1254 |
Nsp3(1-418aa)-R | CGGCCATGGCTACTCTTTTTCAATAGAGGATTTG | ||
NSP3–2 | Nsp3(410-601aa)-F | ACGCGTCGACCGCCAATGTAATGACTAGAGCTG | 1230–1803 |
Nsp3(410-601aa)-R | CGGCCATGGCTAGCTAGGCAACTGGTTTGTAACATC | ||
NSP3–3 | Nsp3(590-1215aa)-F | ACGCGTCGACCGTCTTCGTATACACTGACCAGGAG | 1770–3645 |
Nsp3(590-1215aa)-R | CGGGGTACCCTA CAGAATTTACCAGTACCATTAGC | ||
NSP3–4 | Nsp3(1168-1510aa)-F | ACGCGTCGACCCATATTGTTTTCGCATGCTCAAACCCG | 3504–4510 |
Nsp3(1168-1510aa)-R | CGGGGTACCCTATGAACCACTTTTTGGAGACACAG |