Fig. 7

Nsp3 causes deubiquitination and Nsp3 (590–1215 aa) can inhibit the ubiquitination level of IκBα. a HA-tagged ubiquitin eukaryotic expression plasmid (pCMV-HA-Ub) and the Myc-tagged Nsp3, or its truncated gene fragments, were co-transfected into the IPEC-J2 cells or HEK-293 T cells. At 24 h post-transfection, the cells were treated with 10 μg/mL poly (I:C). After 12 h, the level of protein ubiquitination in cells was detected by western blotting. b IPEC-J2 cells or HEK-293 T cells were co-transfected with pCMV-HA-Ub and Nsp3 (590–1215 aa) eukaryotic expression plasmids. After transfection for 24 h, 10 μg/mL poly (I:C) was added to the cells. After 12 h, the cell lysates were collected for co-immunoprecipitation experiments. Results are representative of three independent experiments