Fig. 4
Effect of Nsp3 on NF-κB-regulated cytokine expression. a HEK-293 T cells or (b) IPEC-J2 cells seeded into 24-well plates at a concentration of 0.5–1.0 × 105 cells/mL were co-transfected with eukaryotic expression plasmid Nsp3 (1 μg) and the empty vector pCMV-HA (1 μg). At 24 h post-transfection, 10 μg/mL poly (I:C) was added to activate the NF-κB signaling pathway, while sterile PBS was used as a negative control. After 12 h, the cell extracts were collected and mRNA levels of IL-1, IL-6, IL-8, TNF-α and β-actin were detected by real-time PCR. β-actin was used as an internal reference gene. Values are the mean ± SD of three independent tests. *P < 0.05 and **P < 0.01 compared with mock infection group