Fig. 3
From: The effects of autophagy on the replication of Nelson Bay orthoreovirus
Effects of autophagy regulators on NBV-MB infection in BHK cells. BHK cells were treated with 3-MA (10 mM) and RAPA (100 nM) for 2 h and then infected with NBV-MB at a MOI of 1. After 12, 24, or 36 h, cellular RNA was extracted and subjected to real-time PCR to measure the level of LC3 mRNA a After 24 h of treatment, LC3 proteins in cell lysates were measured by western blotting b After 24 h of infection, the viral titer in supernatants from samples exposed to each treatment was measured in a plaque assay (c)