Fig. 2

NBV-MB infection and autophagy in BHK cells. a BHK cells were cultured in 6-well plates and infected with the NBV-MB strain of NBV at a MOI of 0.5, 1, or 1.5. After 24 h, samples were collected and subjected to western blotting to detect LC3-I/II and β-actin. b BHK cells were cultured in 6-well plates and infected with NBV-MB at a MOI of 1. Samples were collected at 6, 12, 24, and 36 h post-infection and subjected to western blotting to detect LC3-I/II and β-actin. c BHK cells were transfected with the GFP-LC3 plasmid after 24 h and treated with RAPA (or not) for 2 h, followed by NBV-MB infection. e Integrated density of GFP-LC3 in cells displayed as a histogram. d BHK cells were cultured in 6-well plates and infected with the NBV-MB strain inactivated by heat treatment (70 °C for 30 min). After 24 h, the LC3 protein in cell lysates was measured by western blotting