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Fig. 3 | Virology Journal

Fig. 3

From: A GFP-tagged version of the pseudorabies virus protein UL56 localizes to the Golgi and trans-Golgi network through a predicted C-terminal leucine-rich helix in transfected cells

Fig. 3

The pUL56 induces Golgi-associated protein Rab6a downregulation. a Co-IP assay shows that pUL56 does not interact with Rab6a. b HEK293T cells were co-transfected with plasmid expressing Flag-Rab6a with GFP or GFP-pUL56 (1 μg per plasmid). At 48 hpt, the cell lysates were collected and subjected to Western blot analysis using antibodies against Flag, GFP and β-actin. The optical density ratio of Flag-Rab6a/β-actin in GFP expressing group was set to 1. ****, P < 0.0001

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