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Table 1 Real-time PCR primers and probes specifications

From: Association of polymorphisms in inflammatory cytokines encoding genes with severe cases of influenza A/H1N1 and B in an Iranian population

Primers/Probes

Sequence (5′ > 3′)

Flu A Forward

GACAAAATAACATTCGAAGCAACTGG

Flu A Reverse

GGGAGGCTGGTGTTTATAGCACC

Flu A Probea

GCATTCGCAA″t″GGAAAGAAATGCTGG

Flu B Forward

TCCTCAAYTCACTCTTCGAGCG

Flu B Reverse

CGGTGCTCTTGACCAAATTGG

Flu B Probeb

CCAATTCGAGCAGCTGAAACTGCGGTG

  1. aTaqMan probes are labeled at 5′- end with FAM and at the 3′- end with BHQ1
  2. bTaqMan probes are labeled at 5′- end with ROX and at 3′- end with BHQ1
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Virology Journal

ISSN: 1743-422X