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Fig. 2 | Virology Journal

Fig. 2

From: Entry of Challenge Virus Standard (CVS) -11 into N2a cells via a clathrin-mediated, cholesterol-, dynamin-, pH-dependent endocytic pathway

Fig. 2

Clathrin-1 is required for CVS-11 entry. a CHC knockdown was determined by RT-qPCR. b siCtrl- or siCHC- transfected cells were infected with CVS-11 (MOI 0.1, 0.5, 1). At 24 h p.i., the cell was lysed to determine RABV N RNA copy numbers by RT-qPCR. c, d Results are presented as the means ± SD of data from three independent experiments. Effect of CHC knockdown on CVS-11 infectivity was determined by western blot at 48 h p.i. with anti-RABV, anti-CHC and GAPDH antibodies (c). Relative protein levels were analyzed by using ImageJ (d). Student’s t test, p < 0.05 (*); p < 0.01 (**); p < 0.001 (***)

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