Fig. 3

RNA fluorescent in situ hybridization. Biopsied specimens obtained from the 11 o’clock direction of the cervix. These specimens were diagnosed as SMILE and high-grade squamous intraepithelial lesion (HSIL). a Basal and parabasal SMILE cells were positive by ISH analysis using a probe for HPV type 68 (red: arrows). b All layers in the epithelium of HSIL showed a positive signal by ISH using a probe for HPV type 52 (blue: arrow)