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Table 3 Primers and probes used for RAA and tq-PCR

From: Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7

  Primer/probe Sequence (5′ to 3′) Product size (bp) Source
RAA AdV 3-F ATTCCGGCACAGCTTACAATTCACTCGCTCC 184 This paper
  AdV 3-R TCAGTAGTGG TAATGTCTTT CCCAATTTGC  
  AdV 3-Pa ACAATGCAGTAACTACCACCACAAACACA
[HEXdT][THF][BHQ-dT]GGCATTGCTTCCAT[C3-spacer]
 
  AdV 7-F ACAACGGGAGAAGACAATGCCACCACATACAC 186 Thispaper
  AdV 7-R
AdV 7-Pa
IC-Pa
TCCATCAATATCAGTCCATGATTCTTCTCC
AAGACATTACTGCAGACAACAAGCCCATT
[HEXdT][THF][BHQ-dT]GCCGATAAAACATAT[C3-spacer]
GTAAGGTGCTAGACTAAAATTGTTGGGACTT
[FAMdT]G[THF]A[BHQ-dT]CTCTGAAGTAAAAGG[C3-spacer]
 
tq-PCR F-primer GGYCCYAGYTTYAARCCCTAYTC   [15]
  R-primer AAYTTGAGGYTCTGGYTGATCKG  
  Probe3 HEX-ACAATGCAGTAACTACCACCACAA-MGB  
  Probe7 Cy5-TTACTGCAGACAACAAGCCCAT-MGB  
  1. aProbe modifications: FAM 6-carboxyfuorescein, HEX 5-hexachlorofuorescein, THF Tetrahydrofuran, BHQ Black hole quencher, C3-spacer 3′ phosphate blocker