Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7

Table 3 Primers and probes used for RAA and tq-PCR

	Primer/probe	Sequence (5′ to 3′)	Product size (bp)	Source
RAA	AdV 3-F	ATTCCGGCACAGCTTACAATTCACTCGCTCC	184	This paper
	AdV 3-R	TCAGTAGTGG TAATGTCTTT CCCAATTTGC
	AdV 3-P^a	ACAATGCAGTAACTACCACCACAAACACA [HEXdT][THF][BHQ-dT]GGCATTGCTTCCAT[C3-spacer]
	AdV 7-F	ACAACGGGAGAAGACAATGCCACCACATACAC	186	Thispaper
	AdV 7-R AdV 7-P^a IC-P^a	TCCATCAATATCAGTCCATGATTCTTCTCC AAGACATTACTGCAGACAACAAGCCCATT [HEXdT][THF][BHQ-dT]GCCGATAAAACATAT[C3-spacer] GTAAGGTGCTAGACTAAAATTGTTGGGACTT [FAMdT]G[THF]A[BHQ-dT]CTCTGAAGTAAAAGG[C3-spacer]		Thispaper
tq-PCR	F-primer	GGYCCYAGYTTYAARCCCTAYTC		[15]
	R-primer	AAYTTGAGGYTCTGGYTGATCKG
	Probe3	HEX-ACAATGCAGTAACTACCACCACAA-MGB
	Probe7	Cy5-TTACTGCAGACAACAAGCCCAT-MGB

^aProbe modifications: FAM 6-carboxyfuorescein, HEX 5-hexachlorofuorescein, THF Tetrahydrofuran, BHQ Black hole quencher, C3-spacer 3′ phosphate blocker

ISSN: 1743-422X