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Fig. 3 | Virology Journal

Fig. 3

From: Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7

Fig. 3

A1 Amplification curves 1 to 4 indicate HAdV 3 clinical samples with triplex quantitative real-time PCR (tq-PCR) cycle threshold (CT) values of 16.48, 20.39, 36.42, and 36.45 with 1000 copies of the IC in the HEX detection channel. A2 Amplification curves for 1000 copies of the IC combined with (curves 1 to 4) samples as in A1, in the FAM detection channel. The same color amplification curves in A1 and A2 indicate use of the same pair of primers in the reaction tube. B1 Amplification curves 1 to 4 indicate HAdV 7 clinical samples with tq-PCR CT values of 15.85, 17.65, 33.57, and 35.99 with 1000 copies of the IC in the HEX detection channel. B2 Amplification curves for 1000 copies of the IC combined with (curves 1 to 4) samples as in B1, in the FAM detection channel. The same color amplification curves in B1 and B2 indicate use of the same pair of primers in the reaction tube

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