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Fig. 5 | Virology Journal

Fig. 5

From: A sensitive luminescence syncytium induction assay (LuSIA) based on a reporter plasmid containing a mutation in the glucocorticoid response element in the long terminal repeat U3 region of bovine leukemia virus

Fig. 5

Comparison of LuSIAs using CC81-GREMG and CC81-BLU3G with white blood cells (WBCs) from BLV-infected cows. Infectivity of BLV was assessed by LuSIA using CC81-GREMG with 5 × 105, 1 × 105, or 2 × 104 WBCs collected from seven BLV-infected and two uninfected cows. a CC81-GREMG cells were cultured with or without 1 × 105 WBCs collected from BLV-infected or -uninfected cows. b Correlations between BLV provirus load and the number of syncytia expressing EGFP, when CC81-BLU3G and CC81-GREMG cells were co-cultured with WBCs. Provirus loads were assessed by the CoCoMo-qPCR-2 method

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