TY - JOUR AU - Sato, Hirotaka AU - Watanuki, Sonoko AU - Bai, Lanlan AU - Borjigin, Liushiqi AU - Ishizaki, Hiroshi AU - Matsumoto, Yasunobu AU - Hachiya, Yuma AU - Sentsui, Hiroshi AU - Aida, Yoko PY - 2019 DA - 2019/05/20 TI - A sensitive luminescence syncytium induction assay (LuSIA) based on a reporter plasmid containing a mutation in the glucocorticoid response element in the long terminal repeat U3 region of bovine leukemia virus JO - Virology Journal SP - 66 VL - 16 IS - 1 AB - Bovine leukemia virus (BLV) causes enzootic bovine leukosis, the most common neoplastic disease of cattle. Previously, we reported the luminescence syncytium induction assay (LuSIA), an assay for BLV infectivity based on CC81-BLU3G cells, which form syncytia expressing enhanced green fluorescent protein (EGFP) when co-cultured with BLV-infected cells. To develop a more sensitive LuSIA, we here focused on the glucocorticoid response element (GRE) within the U3 region of the BLV long terminal repeat (LTR). SN - 1743-422X UR - https://doi.org/10.1186/s12985-019-1172-2 DO - 10.1186/s12985-019-1172-2 ID - Sato2019 ER -