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Fig. 4 | Virology Journal

Fig. 4

From: miR-27b-mediated suppression of aquaporin-11 expression in hepatocytes reduces HCV genomic RNA levels but not viral titers

Fig. 4

AQP11 is a crucial factor for HCV genome replication. a knockdown efficiencies of siAQP11. AQP11 mRNA levels were determined 2 days after siRNA transfection. AQP11 mRNA levels were normalized to GAPDH. b HCVcc genome copy numbers in AQP11-knocked down cells. Huh7.5.1 cells were transfected with 50 nM of siAQP11. Two days after siRNA transfection, cells were infected with HCVcc at an MOI of 1. HCVcc genome copy numbers were determined 2 and 6 days after infection. HCVcc genome copy numbers were normalized to GAPDH. The relative HCV genome levels in siControl-treated cells on day 2 were normalized to 1. c cell viabilities following knockdown of AQP11. Huh7.5.1 cells were transfected with siAQP11. Cell viabilities were determined 3 days after transfection. The cell viabilities of the mock group were normalized to 100%. d AQP11 mRNA levels following transfection with pAQP11. AQP11 mRNA levels were determined 2 days after plasmid transfection. AQP11 mRNA levels were normalized to GAPDH. The relative AQP11 mRNA levels in pControl-treated cells were normalized to 1. e HCVcc genome copy numbers in AQP11-overexpressing cells. Huh7.5.1 cells were transfected with 6 μg/ml of pAQP11. Two days after plasmid transfection, cells were infected with HCVcc at an MOI of 0.5. HCVcc genome copy numbers were determined 4 days after infection. The HCVcc genome copy numbers were normalized to GAPDH. Relative HCV genome levels in pControl-treated cells were normalized to 1. f HCV subgenomic replicon levels in Huh7.5.1 1b Feo cells following AQP11 over-expression. Huh7.5.1 1b Feo cells were transfected with 6 μg/ml of pAQP11. HCV subgenomic replicon levels were determined 2 days after transfection. The data are expressed as the means ± S.D. (N = 3), *p < 0.05, **p < 0.01, ***p < 0.001

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