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Fig. 3 | Virology Journal

Fig. 3

From: miR-27b-mediated suppression of aquaporin-11 expression in hepatocytes reduces HCV genomic RNA levels but not viral titers

Fig. 3

Reduction in AQP11 expression in Huh7.5.1 cells following infection with HCVcc. a AQP11 mRNA levels following infection with HCVcc at an MOI of 1. Total RNA was recovered on days 3 and 4, followed by real-time RT-PCR analysis. AQP11 mRNA levels were normalized to GAPDH. The relative AQP11 mRNA levels in mock-infected cells on each day were normalized to 1. The data are expressed as the means ± S.D. (N = 3). *p < 0.05. b AQP11 protein levels following infection with HCVcc. Huh7.5.1 cells were infected with HCVcc at an MOI of 1. Total cell lysates were prepared at the indicated time points, followed by western blot analysis. c AQP11 protein levels in HCV subgenomic replicon-expressing cells. Total cell lysates was prepared from Huh7.5.1 1b Feo cells and Huh7.5.1 cells, followed by western blot analysis. Representative western blot-analysis data from two independent experiments are shown

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