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Fig. 1 | Virology Journal

Fig. 1

From: miR-27b-mediated suppression of aquaporin-11 expression in hepatocytes reduces HCV genomic RNA levels but not viral titers

Fig. 1

Increase in miR-27b expression following infection with HCVcc in Huh7.5.1 cells. a HCVcc genome copy numbers in Huh7.5.1 cells following infection. HCVcc genome copy numbers were normalized to GAPDH. b miR-27b expression levels in Huh7.5.1 cells following infection. Huh7.5.1 cells were infected with HCVcc at an MOI of 1. Total RNA was recovered from the cells at the indicated time points, followed by real-time RT-PCR analysis. miR-27b expression levels were normalized to U6. Expression levels relative to mock-infected cells are shown. c HCVcc genome copy numbers in Huh7.5.1 cells following transfection with miR-27b mimic. HCVcc genome copy numbers were determined by real-time PCR analysis 72 h after infection. HCVcc genome copy numbers were normalized to GAPDH. The relative HCVcc genome copy numbers in the cells transfected with 25 nM of control mimic were normalized to 1. The data are expressed as the means ± S.D. (N = 3). **p < 0.01, ***p < 0.001

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