A candidate DNA vaccine encoding a fusion protein of porcine complement C3d-P28 and ORF2 of porcine circovirus type 2 induces cross-protective immunity against PCV2b and PCV2d in pigs

Table 1 Experimental design

Group	Immunized recombinant plasmid	Dose	Challenge isolate	Challenge dose (TCID₅₀/ml)
pV/PCV2b	pVAX1	400 μg/ml	PCV2b	2 × 10^5.5
pV/PCV2d	pVAX1	400 μg/ml	PCV2d	2 × 10^5.5
pVO/PCV2b	pVAX1-ORF2	400 μg/ml	PCV2b	2 × 10^5.5
pVO/PCV2d	pVAX1-ORF2	400 μg/ml	PCV2d	2 × 10^5.5
pVOC3/PCV2b	pVAX1-ORF2-C3d-P28.3	400 μg/ml	PCV2b	2 × 10^5.5
pVOC3/PCV2d	pVAX1-ORF2-C3d-P28.3	400 μg/ml	PCV2d	2 × 10^5.5
Negative group	PBS	1 ml	/	/

ISSN: 1743-422X