Fig. 4
From: Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization
In vitro cleavage activity of the DHAV 3C protease. The cleavage reactions were performed with different concentrations of substrate. The enzymatic activity of the DHAV 3C protease was determined by incubation with (a) 10 μM, (b) 100 μM, (c) 150 μM, (d) 200 μM, (e) 250 μM, and (f) 300 μM substrate. All reactions were determined in triplicate and kinetic parameters were calculated using GraphPad Prism 5