Fig. 2From: Biochemical characterization of recombinant Avihepatovirus 3C protease and its localizationOptimization of 3C protease expression. The DHAV 3C protease was expressed at different (a) IPTG concentrations (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, and 1.4 mM), (b) temperatures (16, 30, and 37 °C), and (c) induction times (2, 4, 6, 8, 10, and 12 h). (d) The bacterial lysates were analyzed for the presence of the DHAV 3C protease by immunoblottingBack to article page