Skip to main content

Table 1 The primers were used to construct the various truncated, internal deletion mutants by PCR

From: Identification of nuclear localization signal and nuclear export signal of VP1 from the chicken anemia virus and effects on VP2 shuttling in cells

Primer name

Type

Length

Sequence (5′-3′)

VP1 EcoR1

Forward

25-mer

AGAATTCATGGCAAGACGAGCTCGC

VP1 Xho1 R

Reverse

20-mer

TCCTCGAGGGGCTGCGTCTC

VP1 ND9 EcoR1

Forward

24-mer

AGAATTCATGGGCCGATTTTACGC

VP1 DN19 EcoR1

Forward

26-mer

AGAATTCATGCACAACCTCAAGCGAC

VP1 ND30 EcoR1

Forward

25-mer

AGAATTCAAATTTCGCCATCGCCGC

VP1 ND60 EcoR1

Forward

25-mer

AGAATTCCTGCCGAACCCGCAGAGC

VP1 ND129 EcoR1

Forward

25-mer

AGAATTCGGCGAACTGATTGCGGAT

VP1 CD10 Xho1

Reverse

21-mer

TCCTCGAGGCCTCTCGGTCTG

VP1 CD103 Xho1

Reverse

24-mer

TCCTCGAGGGCGACTCTCGGCCCC TAAGATGG

VP1 CD126 Xho1

Reverse

22-mer

TCTCGAGTCCGATTTTGCTCAC

VP1 internal NES deletion 1162

Forward

24-mer

GCGGCCGCGGCACAAATAAGTCGC

VP1 internal NES deletion 1119

Reverse

27-mer

GCGGCCGCATGATGCGGGTGCACT