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Fig. 3 | Virology Journal

Fig. 3

From: Identification of nuclear localization signal and nuclear export signal of VP1 from the chicken anemia virus and effects on VP2 shuttling in cells

Fig. 3

NLS motif characterized by multiple-site fragment deletions. a Truncated fragments of VP1 (gray bars) encoding constructs fused with GFP (oval shape) were used in this study, including VP1-ND129, VP1-ND60, VP1-ND30, VP1-ND19, VP1-ND9 and VP1-CD10. The putative NLS1 (black box) and NLS2 (gray box) are shown in the full length of VP1 (gray bar). The numbers of amino acid residues are presented in the constructs. b Subcellular localization of N-terminal deleted VP1-GFP constructions (VP1-ND129, VP1-ND60, VP1-ND30, VP1-ND19 and VP1-ND9) and C-terminal deleted VP1-CD10 are shown in CHO cells. All cells were fixed and stained with DAPI. The distributions of the truncated VP1-GFP constructs in the cells were observed using fluorescence microscopy (400× magnification)

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