Fig. 1
From: Phylogenetic analysis and phenotypic characterisatics of two Tibet EV-C96 strains
Real-time RT-PCR curve and temperature sensitivity test curve of two Tibetan EV-C96 strains. a Enterovirus RNAs in RD, Hep-2 and Hela cell cultures were detected with a real time RT-PCR-based enterovirus screening method. A Xinjiang EV-B85 strains, HYTY-ARL-AFP02F, was used as positive control, the RD cell culture without inoculating samples was used as negtive control. b. Strain 2005-T49 is a non-temperature sensitive (Non-Ts) strain. Two Xinjiang EV-B85 strains, HYTY-ARL-AFP02F, which is not temperature sensitive, and HT-LYKH202F, which is temperature sensitive (Ts), were used as controls. The blue and red lines show the growth curves of the viruses in RD cells at 36 °C and 39.5 °C, respectively