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Table 6 Evaluation of the clinical performance of the multiplex RT-qPCR assay for the detection and genotyping of equine rotavirus A in fecal samples compared to VP7-specific RT-PCR and sequencing (gold standard). (a) NSP3 (b) G3 VP7 and (c) G14 VP7

From: Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

a
  VP7-specific RT-PCR
  Positive Negative Total
NSP3-specific RT-qPCR Positive 85 0 85
Negative 0 92 92
Total 85 92 177
b
  ERVA genotype G31
  Positive Negative Total
G3-specific RT-qPCR Positive 38 0 38
Negative 3 136 139
Total 41 136 177
c
  ERVA genotype G141
  Positive Negative Total
G14-specific RT-qPCR Positive 44 1 45
Negative 0 132 132
Total 44 133 177
  1. 1Genotype determined by Sanger sequencing