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Table 2 Primers used for RT-PCR amplification and sequencing of VP7 (genome segment 9) of ERVA

From: Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

Primer name

Target

Nucleotide Position

Sequence (5′ to 3′)

Application

RVAVP7-Gra-5

VP7

1-20a

GGCTTTAAAAGCGAGAATTT

RT-PCR and sequencing

RVAVP7-Gra-3

VP7

1062–1,044a

GGTCACATCATACAACTCT

RT-PCR and sequencing

RVAVP7–389-R

VP7

389-370a

CCAGTAGGCCATCCTTTAGT

Sequencing

RVAVP7-635-F

VP7

635-659a

GTCCACTTAATACACAAACTCTAGG

Sequencing

RVAVP7-241-R

VP7

245-220a

GCAGTRTCCATTGAACCAGTAATTG

Sequencing

RVAVP7-852-F

VP7

856-879a

GAYATAACGGCTGATCCAACTACG

Sequencing

RVAVP7-881-F

VP7

885-906a

CTCCACAGATTGGACGAATGA

Sequencing

  1. anucleotide position based on GenBank Accession number KM454508.1