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Table 1 Characteristics of alfalfa-associated nucleorhabdovirus (AaNV) encoded proteins [sizes in amino acids (aa), molecular masses (MW) in kilo Dalton (kDa), the isoelectric points (IEP), predicted cell nuclear localisation signals (cNLS) and nuclear export signals (NES)]

From: Characterisation of a novel nucleorhabdovirus infecting alfalfa (Medicago sativa)

Putative gene function

Gene

Size

(aa)

MW

(kDa)

pairwise aa sequence identity (%)

IEP

Predicted NLS

NES position

BCaRV-1

DYVV

Position

Partite

aa sequence

cNLS Mapper Score

Predicted location

Nucleocapsid protein

N

443

50.3

33.5

36.6

7.1

408

Bic

RAGIKRQAGDHETQGTKRARTS

12.7

eN

351

Phosphoprotein

P

363

41.1

11.5

14

6.4

146

Monod

RGNKRRKRSD

10

eN

ND

Putative cell-to-cell movement protein

P3

322

37.2

14.6

22.4

8.6

17

Bi

PTKKRTSQDKYNFRSTESLYAEPYNKIIRTK

7

pN

ND

Matrix protein

M

277

31.4

14.9

16.5

7.6

213

Bi

RSSVKITGKQMRARSSSRSRSPYKVSLSSNKRTYLD

4.3

N/Cp

136

Unknown protein

U

113

12.4

NAa

NA

4.0

NDb

ND

ND

ND

NA

31

Glycoprotein

G

629

71.6

26.4

24.6

7.3

443

Bi

KSAYKKKLPYEVTAWNGDKIMSEYPYKNIVVE

6

pN

ND

RNA-dependent RNA polymerase

L

2038

234.8

35.8

36.7

7.8

780

Bi

EKTAIKRRMRAFRDDLGQKMKKR

10

eN

88,90 and 149

  1. The amino acid sequence identities between the putative gene products of AaNV and those of black currant-associated rhabdovirus 1 (BCaRV-1; MF543022) and datura yellow vein virus (DYVV; NC_028231). The predicted protein cell nuclear localisation signal (cNLS; http://nls-mapper.iab.keio.ac.jp/cgi-bin/NLS_Mapper_form.cgi) Mapper score and Nuclear export signals (NES) are also mentioned. The cut-off values of the cNLS mapper scores: 8, 9, or 10 = the protein is predicted to be exclusively localised to the nucleus (eN), 6, 7 or 8 = partially localised to the nucleus (pN), 3, 4, or 5 = localised to both the nucleus and the cytoplasm (N/Cp), and 1 or 2 = localised to the cytoplasm (Cp). aNA: not applicable. bND: not detectable. cBi: Predicted bipartite NLSd Mono: Predicted monopartite NLS