Table 1 The ISG primers and probes used for real-time PCR
Gene | Sequence of primers and probes |
|---|---|
GADPH | F: GAAGGTGAAGGTCGGAGTC R: GAAGATGGTGATGGGATTTC P: FAM-AAGGTCGGAGTCAACGGATTTGGTC-TAMRA |
ISG15 | F: GGACAAATGCGACGAACCTC R: GCTCACTTGCTGCTTCAGG P: FAM-CCCGCCAGCATCTTCACCGT-TAMRA |
MX1 | F: TGCAGGACAAGGACACCTAC R: TCACCACGGCTAACGGATAA P: FAM-ATAAAGCCCAGAATGCCATCGCC-TAMRA |
OAS1 | F: AGCATTTTCCGTGAAGTTTG R: GGGTTAGGTTTATAGCCGCC P: FAM-AGAGGCCGATCTGACGCTGAC-TAMRA |
IFITM3 | F: GAGAACCATCCCAGTAACCC R: GACAGGAGAGAAGAAGGTTTGG P: FAM-TTCGCTGGACACCATGAATCACACT-TAMRA |