Fig. 5From: Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell culturesEffect of rhIFN treatment on ISG induction in different cells. After treatment of A549 cells, 2D HAE cells, and 3D HAE cells with rhIFN-α2b or rhIFN-λ1, the total RNA was extracted and reverse transcribed. The relative expression levels of ISG15, MX1, OAS1, and IFITM3 were measured using Taqman real-time PCR assays and quantified by the 2−ΔΔCT method. *P < 0.05; **P < 0.01Back to article page