Fig. 4

PSV infection occurs in a dynamin-dependent manner. a-b Effect of pretreatment of IPEC-J2 cells with increasing concentrations of dynasore on PSV titer as determined by TCID₅₀ or virus VP1 protein synthesis as detected by western blot. The horizontal line shows the subtoxic concentrations of dynasore on IPEC-J2 cells. c IPEC-J2 cells were transfected with plasmids encoding wild-type dynamin-GFP (Dyn-WT) or the DN mutant dynamin (K44A) (Dyn-DN). At 30 h after transfection, cells were infected with PSV and quantified PSV VP1 protein expression was performed by western blot. Data are presented as means ± SD. The error bars indicate the standard deviations of triplicate samples from one of two independent experiments