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Fig. 4 | Virology Journal

Fig. 4

From: Entry of sapelovirus into IPEC-J2 cells is dependent on caveolae-mediated endocytosis

Fig. 4

PSV infection occurs in a dynamin-dependent manner. a-b Effect of pretreatment of IPEC-J2 cells with increasing concentrations of dynasore on PSV titer as determined by TCID50 or virus VP1 protein synthesis as detected by western blot. The horizontal line shows the subtoxic concentrations of dynasore on IPEC-J2 cells. c IPEC-J2 cells were transfected with plasmids encoding wild-type dynamin-GFP (Dyn-WT) or the DN mutant dynamin (K44A) (Dyn-DN). At 30 h after transfection, cells were infected with PSV and quantified PSV VP1 protein expression was performed by western blot. Data are presented as means ± SD. The error bars indicate the standard deviations of triplicate samples from one of two independent experiments

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