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Fig. 1 | Virology Journal

Fig. 1

From: Entry of sapelovirus into IPEC-J2 cells is dependent on caveolae-mediated endocytosis

Fig. 1

Endosomal acidification is required for PSV infection of IPEC-J2 cells. a and b IPEC-J2 cells were mock-treated or pre-treated with different concentrations of NH4Cl or chloroquine (CQ) at 37 °C for 1 h, and then the cells were inoculated with PSV (MOI of 0.5). At 24 hpi, infected cells were collected and virus titers were determined. The horizontal line shows results of subtoxic concentrations of NH4Cl or CQ on cells as determined by cell viability assay. Data are presented as means ± SD. The error bars indicate the standard deviations of triplicate samples from one of two independent experiments. c PSV protein synthesis was suppressed by pretreatment NH4Cl or CQ and in a concentration-dependent manner. At 24 h after virus infection, cell lysates were collected and the expression level of the viral major VP1 protein was measured by western blotting. *P < 0.05; **P < 0.01

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