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Table 1 Primers used for site-directed mutagenesis of PVX mutants

From: Evaluation of Potato virus X mild mutants for cross protection against severe infection in China

Mutant Primer Sequences
V45R Forward 5′-CTCAAACGCGTGAAGCGGCTAATGATCTAGAGGGGTTCGGCATAG-3′
Reverse 5′-GCCGCTTCACGCGTTTGAGCGTACGGGTTAGATAGTTTGTGTTTTTCC-3′
E46A Forward 5′-TCAAACGGTTGCCGCGGCTAATGATCTAGAGGGGTTCGGCATAGC-3′
Reverse 5′-ATTAGCCGCGGCAACCGTTTGAGCGTACGGGTTAGATAGTTTGTGTTTTTC-3′
T140A Forward 5′-ACAGAGATCGCCACGGACACAGCATACATTAGTGACACTCTGCACTTC-3′
Reverse 5′-GTGTCCGTGGCGATCTCTGTGAGCTTGTCTATTATTGTTTCCTTTGGG-3′
D200A Forward 5′-TACTTTGGAGCCGGTTTCCAGTATATACCAGGCAACCATGGTGG-3′
Reverse 5′-TACTGGAAACCGGCTCCAAAGTATTTGAGGCTGTATATGTTCGGGT-3′
N243A Forward 5′-CTCGGACATCTCGCCTACACGACTGAGCAGGTTGAGATGCACACAG-3′
Reverse 5′-AGTCGTGTAGGCGAGATGTCCGAGAAAGCTATCCTTGGGGTCCCTC-3′
K299A Forward 5′-ATCTTTCTCCCGGCAGTTCACAACTGCAAGAAGCCGATTCTGAAGA-3′
Reverse 5′-GTTGTGAACTGCCGGGAGAAAGATCTGTGGTGGAATCACATACCTGTCA-3′
V300R Forward 5′-CTCCCGAAACGCCACAACTGCAAGAAGCCGATTCTGAAGAAAAC-3′
Reverse 5′-CAGTTGTGGCGTTTCGGGAGAAAGATCTGTGGTGGAATCACATACC-3′
S341R Forward 5′-ATTAAATCGCGCGACTTGGACAAATACTCTGCTGTGGAACTGGTTTAC-3′
Reverse 5′-GTCCAAGTCGCGCGATTTAATTAATTGTCTGACTTTGGCAAAAATG-3′
D344A Forward 5′-GTCTGACTTGGCGAAATACTCTGCTGTGGAACTGGTTTACTTAGTGAGC-3′
Reverse 5′-GCAGAGTATTTCGCCAAGTCAGACGATTTAATTAATTGTCTGACTTTGGCA-3′
E446A Forward 5′-TCGGACGTAGCCGAAATGGAAAGTTTGTTCTCAGATGGGGACCTG-3′
Reverse 5′-TTTCCATTTCGGCTACGTCCGACACTTCCCTTGGTCGGAAGG-3′
A494K Forward 5′-ATTAAAGAACCTAAAGGAGACAGAAATCAATACTCAAACCCTGCGGAA-3′
Reverse 5′-TTCTGTCTCCTTTAGGTTCTTTAATTCCTTGACCGACATCCATCTCA-3′
K525A Forward 5′-CAGACCACAGCGAAGGCTAAGCGCCTAGCTGAAATCCAGGAGTCC-3′
Reverse 5′-GCTTAGCCTTCGCTGTGGTCTGATGTTTCACCTCTCTACTGTGTTTCCTGT-3′
K526A Forward 5′-CAGACCACAAAGGCGGCTAAGCGCCTAGCTGAAATCCAGGAGTCC-3′
Reverse 5′-GCTTAGCCGCCTTTGTGGTCTGATGTTTCACCTCTCTACTGTGTTTCCTGT-3′
K528A Forward 5′-AAAGAAGGCTGCGCGCCTAGCTGAAATCCAGGAGTCCATGA-3′
Reverse 5′-TAGGCGCGCAGCCTTCTTTGTGGTCTGATGTTTCACCTCTCTACTG-3′
E540A Forward 5′-ATGAGAGCAGCCGGTGAAGCTGAATCAAATGAGATGAGCGGG-3′
Reverse 5′-AGCTTCACCGGCTGCTCTCATGGACTCCTGGATTTCAGCTAGGC-3′
S545A Forward 5′-GAAGCAAATGAGATGAGCGGGGGCATGGGGGCAATACCG-3′
Reverse 5′-CCCGCTCATCTCATTTGCTTCAGCTTCACCTTCTGCTCTCATGGACT-3′
S565A Forward 5′-AGCACGGCTGATGCTAGACAAGAACTCACACTCCCAACCACC-3′
Reverse 5′-GTCTAGCATCAGCCGTGCTGGGAAGTTCAGCGTTGCTCG-3′
D566A Forward 5′-AGCACGAGTGCCGCTAGACAAGAACTCACACTCCCAACCACCAA-3′
Reverse 5′-TCTTGTCTAGCGGCACTCGTGCTGGGAAGTTCAGCGTTGCTCG-3′
M610K Forward 5′-GAGACAGCAAAACAACAAGTCATCGAAGGACTCCCTTGGAAA-3′
Reverse 5′-ACTTGTTGTTTTGCTGTCTCAACAGCTTCTTTTCCAGGGAGCTT-3′
K620A Forward 5′-ACTCCCTTGGGCACACTGGATTCCTCAACTAAACGCTGTTGGATTC-3′
Reverse 5′-AATCCAGTGTGCCCAAGGGAGTCCTTCGATGACTTGTTGCATTG-3′
N641A Forward 5′-GGGATAGGGCTGGAACAATGATCATGCCTATCACAGAAATGG-3′
Reverse 5′-ATTGTTCCAGCCCTATCCCTCTGAATTTCTAGCGCCTTGAATC-3′
E662A Forward 5′-ACTTCCCGGCCGGAACTCCAAAAGAGTTGGCACGAGAATTGCTC-3′
Reverse 5′-TGGAGTTCCGGCCGGGAAGTCCTCTTTTTCCAACCCGGAGA-3′
N676A Forward 5′-TGCTCGCTATGGCGAGAAGTCCTGCCACCATCCCTTTGGAC-3′
Reverse 5′-GGACTTCTCGCCATAGCGAGCAATTCTCGTGCCAACTCTTTTGGA-3′
K773A Forward 5′-AGATTGGAGTGCGAAAGTGCCCAACACTGAACCATACATGTTCAAGA-3′
Reverse 5′-GGCACTTTCGCACTCCAATCTAGCCGCAGTTCATTGGTCG-3′
N777A Forward 5′-AAAGTGCCCGCGACTGAACCATACATGTTCAAGACCTATGAAAAGGCAT-3′
Reverse 5′-GGTTCAGTCGCGGGCACTTTCTTACTCCAATCTAGCCGCAGTTC-3′
E779A Forward 5′-CCAACACTGCCCCATACATGTTCAAGACCTATGAAAAGGCATTAATTGG-3′
Reverse 5′-AACATGTATGGGGCAGTGTTGGGCACTTTCTTACTCCAATCTAGCCG-3′
N863A Forward 5′-CGATACTATCTCGCCGCCACACACCGCAACAAGAAAGACCTTGC-3′
Reverse 5′-TGTGTGGCGGCGAGATAGTATCGGCAGTATTTTGAGAAGTACTCTGTCGC-3′
N917A Forward 5′-ACCGGAAGGGCCGACACGTTCACATACGCTGGATGCCAAG-3′
Reverse 5′-ACGTGTCGGCCCTTCCGGTGCCCATGTACAGCTTTCTCTTTTCA-3′
N964A Forward 5′-CACTTCGTGGCGACAAGTGCAAACTCTTCGGCCTTCTGGGAA-3′
Reverse 5′-TGCACTTGTCGCCACGAAGTGAATCCTGTCGGTAGCTCTAGAGAGTGC-3′
N968A Forward 5′-CACAAGTGCAGCGTCTTCGGCCTTCTGGGAAAAGTTAGACA GCACCC-3′
Reverse 5′-GCCGAAGACGCTGCACTTGTGTTCACGAAGTGAATCCTGTCGGT-3′
E1001A Forward 5′-AGCCGGCAGCCGTAGAGCCAATTCGAGAGCCTGAGCCC-3′
Reverse 5′-GCTCTACGGCTGCCGGCTCGTACTCCTTGAGTGCTTGTTCTCTC-3′
R1006A Forward 5′-AGCCAATTGCCGAGCCTGAGCCCCAAACACACATGTGTGT-3′
Reverse 5′-TCAGGCTCGGCAATTGGCTCTACCTCTGCCGGCTCGTACTC-3′
E1041A Forward 5′-ATCCACTCTGCCTCCCATGGCCATTCAAACTGTGTCCAAACTGA-3′
Reverse 5′-CCATGGGAGGCAGAGTGGATCTCTCTGTCAAACTTTTCCAAAAGTTCC-3′
S1042A Forward 5′-ACTCTGAAGCGCATGGCCATTCAAACTGTGTCCAAACTGAAGACACA-3′
Reverse 5′-AATGGCCATGCGCTTCAGAGTGGATCTCTCTGTCAAACTTTTCCAAAAG-3′
K1065A Forward 5′-TCAACAAGCAGCGGATGAGACCCTCCTCTGGGCGACCATAGATG-3′
Reverse 5′-GGTCTCATCCGCTGCTTGTTGATGCGAAAA CAGCTGGATGGTT-3′
S1092A Forward 5′-AATTCTTGGCCAAGAAGGACATTGGAGACGTCCTGTTTCTAAAC-3′
Reverse 5′-GTCCTTCTTGGCCAAGAATTCTCGGAAGTTTGTTTCTTGATTG-3′
N1143A Forward 5′-AACTTGATTGCGGGGACTGTGAGACAGAGCCCAGACTTTGAT-3′
Reverse 5′-CACAGTCCCCGCAATCAAGTTGCACTTTGACTTACTGAGGTATTTG-3′
D1348A Forward 5′-ACAGGCTTGAAGCGAAATTACTCCTCAAGTCGAAGCCTGTAATCACG-3′
Reverse 5′-GGAGTAATTTCGCTTCAAGCCTGTGGAAACTAAGCTTCACTTCTGGAACG-3′
K1427A Forward 5′-TTGTTCGATGAGGCGCAGTGTCAGGCACATACACTCACTTGCAG-3′
Reverse 5′-ACACTGCGCCTCATCGAACAAGTCATGCAGAGAGTCCTTGTGGT-3′
L1456K Forward 5′-CAGAAACTTTAAGTAACCGTTAAGTTACCTTAGAGATTTGAATAAGATGG-3′
Reverse 5′-TAACGGTTACTTAAAGTTTCTGAGGCGGGGAAGTGAGACAGTGCCT-3′
  1. Mutated nucleotides are shown in bold type