Table 1 Primers used for site-directed mutagenesis of PVX mutants
From: Evaluation of Potato virus X mild mutants for cross protection against severe infection in China
Mutant | Primer | Sequences |
|---|---|---|
V45R | Forward | 5′-CTCAAACGCGTGAAGCGGCTAATGATCTAGAGGGGTTCGGCATAG-3′ |
Reverse | 5′-GCCGCTTCACGCGTTTGAGCGTACGGGTTAGATAGTTTGTGTTTTTCC-3′ | |
E46A | Forward | 5′-TCAAACGGTTGCCGCGGCTAATGATCTAGAGGGGTTCGGCATAGC-3′ |
Reverse | 5′-ATTAGCCGCGGCAACCGTTTGAGCGTACGGGTTAGATAGTTTGTGTTTTTC-3′ | |
T140A | Forward | 5′-ACAGAGATCGCCACGGACACAGCATACATTAGTGACACTCTGCACTTC-3′ |
Reverse | 5′-GTGTCCGTGGCGATCTCTGTGAGCTTGTCTATTATTGTTTCCTTTGGG-3′ | |
D200A | Forward | 5′-TACTTTGGAGCCGGTTTCCAGTATATACCAGGCAACCATGGTGG-3′ |
Reverse | 5′-TACTGGAAACCGGCTCCAAAGTATTTGAGGCTGTATATGTTCGGGT-3′ | |
N243A | Forward | 5′-CTCGGACATCTCGCCTACACGACTGAGCAGGTTGAGATGCACACAG-3′ |
Reverse | 5′-AGTCGTGTAGGCGAGATGTCCGAGAAAGCTATCCTTGGGGTCCCTC-3′ | |
K299A | Forward | 5′-ATCTTTCTCCCGGCAGTTCACAACTGCAAGAAGCCGATTCTGAAGA-3′ |
Reverse | 5′-GTTGTGAACTGCCGGGAGAAAGATCTGTGGTGGAATCACATACCTGTCA-3′ | |
V300R | Forward | 5′-CTCCCGAAACGCCACAACTGCAAGAAGCCGATTCTGAAGAAAAC-3′ |
Reverse | 5′-CAGTTGTGGCGTTTCGGGAGAAAGATCTGTGGTGGAATCACATACC-3′ | |
S341R | Forward | 5′-ATTAAATCGCGCGACTTGGACAAATACTCTGCTGTGGAACTGGTTTAC-3′ |
Reverse | 5′-GTCCAAGTCGCGCGATTTAATTAATTGTCTGACTTTGGCAAAAATG-3′ | |
D344A | Forward | 5′-GTCTGACTTGGCGAAATACTCTGCTGTGGAACTGGTTTACTTAGTGAGC-3′ |
Reverse | 5′-GCAGAGTATTTCGCCAAGTCAGACGATTTAATTAATTGTCTGACTTTGGCA-3′ | |
E446A | Forward | 5′-TCGGACGTAGCCGAAATGGAAAGTTTGTTCTCAGATGGGGACCTG-3′ |
Reverse | 5′-TTTCCATTTCGGCTACGTCCGACACTTCCCTTGGTCGGAAGG-3′ | |
A494K | Forward | 5′-ATTAAAGAACCTAAAGGAGACAGAAATCAATACTCAAACCCTGCGGAA-3′ |
Reverse | 5′-TTCTGTCTCCTTTAGGTTCTTTAATTCCTTGACCGACATCCATCTCA-3′ | |
K525A | Forward | 5′-CAGACCACAGCGAAGGCTAAGCGCCTAGCTGAAATCCAGGAGTCC-3′ |
Reverse | 5′-GCTTAGCCTTCGCTGTGGTCTGATGTTTCACCTCTCTACTGTGTTTCCTGT-3′ | |
K526A | Forward | 5′-CAGACCACAAAGGCGGCTAAGCGCCTAGCTGAAATCCAGGAGTCC-3′ |
Reverse | 5′-GCTTAGCCGCCTTTGTGGTCTGATGTTTCACCTCTCTACTGTGTTTCCTGT-3′ | |
K528A | Forward | 5′-AAAGAAGGCTGCGCGCCTAGCTGAAATCCAGGAGTCCATGA-3′ |
Reverse | 5′-TAGGCGCGCAGCCTTCTTTGTGGTCTGATGTTTCACCTCTCTACTG-3′ | |
E540A | Forward | 5′-ATGAGAGCAGCCGGTGAAGCTGAATCAAATGAGATGAGCGGG-3′ |
Reverse | 5′-AGCTTCACCGGCTGCTCTCATGGACTCCTGGATTTCAGCTAGGC-3′ | |
S545A | Forward | 5′-GAAGCAAATGAGATGAGCGGGGGCATGGGGGCAATACCG-3′ |
Reverse | 5′-CCCGCTCATCTCATTTGCTTCAGCTTCACCTTCTGCTCTCATGGACT-3′ | |
S565A | Forward | 5′-AGCACGGCTGATGCTAGACAAGAACTCACACTCCCAACCACC-3′ |
Reverse | 5′-GTCTAGCATCAGCCGTGCTGGGAAGTTCAGCGTTGCTCG-3′ | |
D566A | Forward | 5′-AGCACGAGTGCCGCTAGACAAGAACTCACACTCCCAACCACCAA-3′ |
Reverse | 5′-TCTTGTCTAGCGGCACTCGTGCTGGGAAGTTCAGCGTTGCTCG-3′ | |
M610K | Forward | 5′-GAGACAGCAAAACAACAAGTCATCGAAGGACTCCCTTGGAAA-3′ |
Reverse | 5′-ACTTGTTGTTTTGCTGTCTCAACAGCTTCTTTTCCAGGGAGCTT-3′ | |
K620A | Forward | 5′-ACTCCCTTGGGCACACTGGATTCCTCAACTAAACGCTGTTGGATTC-3′ |
Reverse | 5′-AATCCAGTGTGCCCAAGGGAGTCCTTCGATGACTTGTTGCATTG-3′ | |
N641A | Forward | 5′-GGGATAGGGCTGGAACAATGATCATGCCTATCACAGAAATGG-3′ |
Reverse | 5′-ATTGTTCCAGCCCTATCCCTCTGAATTTCTAGCGCCTTGAATC-3′ | |
E662A | Forward | 5′-ACTTCCCGGCCGGAACTCCAAAAGAGTTGGCACGAGAATTGCTC-3′ |
Reverse | 5′-TGGAGTTCCGGCCGGGAAGTCCTCTTTTTCCAACCCGGAGA-3′ | |
N676A | Forward | 5′-TGCTCGCTATGGCGAGAAGTCCTGCCACCATCCCTTTGGAC-3′ |
Reverse | 5′-GGACTTCTCGCCATAGCGAGCAATTCTCGTGCCAACTCTTTTGGA-3′ | |
K773A | Forward | 5′-AGATTGGAGTGCGAAAGTGCCCAACACTGAACCATACATGTTCAAGA-3′ |
Reverse | 5′-GGCACTTTCGCACTCCAATCTAGCCGCAGTTCATTGGTCG-3′ | |
N777A | Forward | 5′-AAAGTGCCCGCGACTGAACCATACATGTTCAAGACCTATGAAAAGGCAT-3′ |
Reverse | 5′-GGTTCAGTCGCGGGCACTTTCTTACTCCAATCTAGCCGCAGTTC-3′ | |
E779A | Forward | 5′-CCAACACTGCCCCATACATGTTCAAGACCTATGAAAAGGCATTAATTGG-3′ |
Reverse | 5′-AACATGTATGGGGCAGTGTTGGGCACTTTCTTACTCCAATCTAGCCG-3′ | |
N863A | Forward | 5′-CGATACTATCTCGCCGCCACACACCGCAACAAGAAAGACCTTGC-3′ |
Reverse | 5′-TGTGTGGCGGCGAGATAGTATCGGCAGTATTTTGAGAAGTACTCTGTCGC-3′ | |
N917A | Forward | 5′-ACCGGAAGGGCCGACACGTTCACATACGCTGGATGCCAAG-3′ |
Reverse | 5′-ACGTGTCGGCCCTTCCGGTGCCCATGTACAGCTTTCTCTTTTCA-3′ | |
N964A | Forward | 5′-CACTTCGTGGCGACAAGTGCAAACTCTTCGGCCTTCTGGGAA-3′ |
Reverse | 5′-TGCACTTGTCGCCACGAAGTGAATCCTGTCGGTAGCTCTAGAGAGTGC-3′ | |
N968A | Forward | 5′-CACAAGTGCAGCGTCTTCGGCCTTCTGGGAAAAGTTAGACA GCACCC-3′ |
Reverse | 5′-GCCGAAGACGCTGCACTTGTGTTCACGAAGTGAATCCTGTCGGT-3′ | |
E1001A | Forward | 5′-AGCCGGCAGCCGTAGAGCCAATTCGAGAGCCTGAGCCC-3′ |
Reverse | 5′-GCTCTACGGCTGCCGGCTCGTACTCCTTGAGTGCTTGTTCTCTC-3′ | |
R1006A | Forward | 5′-AGCCAATTGCCGAGCCTGAGCCCCAAACACACATGTGTGT-3′ |
Reverse | 5′-TCAGGCTCGGCAATTGGCTCTACCTCTGCCGGCTCGTACTC-3′ | |
E1041A | Forward | 5′-ATCCACTCTGCCTCCCATGGCCATTCAAACTGTGTCCAAACTGA-3′ |
Reverse | 5′-CCATGGGAGGCAGAGTGGATCTCTCTGTCAAACTTTTCCAAAAGTTCC-3′ | |
S1042A | Forward | 5′-ACTCTGAAGCGCATGGCCATTCAAACTGTGTCCAAACTGAAGACACA-3′ |
Reverse | 5′-AATGGCCATGCGCTTCAGAGTGGATCTCTCTGTCAAACTTTTCCAAAAG-3′ | |
K1065A | Forward | 5′-TCAACAAGCAGCGGATGAGACCCTCCTCTGGGCGACCATAGATG-3′ |
Reverse | 5′-GGTCTCATCCGCTGCTTGTTGATGCGAAAA CAGCTGGATGGTT-3′ | |
S1092A | Forward | 5′-AATTCTTGGCCAAGAAGGACATTGGAGACGTCCTGTTTCTAAAC-3′ |
Reverse | 5′-GTCCTTCTTGGCCAAGAATTCTCGGAAGTTTGTTTCTTGATTG-3′ | |
N1143A | Forward | 5′-AACTTGATTGCGGGGACTGTGAGACAGAGCCCAGACTTTGAT-3′ |
Reverse | 5′-CACAGTCCCCGCAATCAAGTTGCACTTTGACTTACTGAGGTATTTG-3′ | |
D1348A | Forward | 5′-ACAGGCTTGAAGCGAAATTACTCCTCAAGTCGAAGCCTGTAATCACG-3′ |
Reverse | 5′-GGAGTAATTTCGCTTCAAGCCTGTGGAAACTAAGCTTCACTTCTGGAACG-3′ | |
K1427A | Forward | 5′-TTGTTCGATGAGGCGCAGTGTCAGGCACATACACTCACTTGCAG-3′ |
Reverse | 5′-ACACTGCGCCTCATCGAACAAGTCATGCAGAGAGTCCTTGTGGT-3′ | |
L1456K | Forward | 5′-CAGAAACTTTAAGTAACCGTTAAGTTACCTTAGAGATTTGAATAAGATGG-3′ |
Reverse | 5′-TAACGGTTACTTAAAGTTTCTGAGGCGGGGAAGTGAGACAGTGCCT-3′ |