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Table 3 Summary results for in vivo challenge experiments

From: The glycoprotein, non-virion protein, and polymerase of viral hemorrhagic septicemia virus are not determinants of host-specific virulence in rainbow trout

Treatment Group Individual tank final CPMa Ave. final CPM Mean day to death±SDb Day 7 infection, # pos/# tested (# > inoculum)c
Experiment 1
 wt VHSVmi 6, 0, 12 6 13.5* 8/9 (5)
 rVHSVmi 0, 0, 12 4 19* 7/9 (3)
 rVHSVmi-Gdk 12, 0, 6 6 14.5* 2/9 (0)
 rVHSVmi-NVdk 0, 24, 6 10 11.6* 6/9 (3)
 rVHSVmi-GNVdk 6, 0, 6 4 12.5* 4/9 (2)
 wt VHSVdk 100 100 4.6* nt
 rVHSVdk 100, 100, 100 100 4.9 ± 0.20 nt
 rVHSVdk-Gmi 100, 100, 100 100 5.2 ± 0.20 nt
 rVHSVdk-NVmi 100, 100, 100 100 4.6 ± 0.28 nt
 rVHSVdk-GNVmi 100, 100, 100 100 4.1 ± 0.15 nt
 mock 0, 0 0 na 0/6
Experiment 2
 wt VHSVmi 0 0 na nt
 rVHSVmi 0, 6, 6 4 8.5* 3/9 (3)
 rVHSVmi-Gdk 0, 0 0 na 2/6 (1)
 rVHSVmi-GNVLdk 0, 12, 0 0 14.5* 3/9 (3)
 wt VHSVdk 100 100 3.5* nt
 rVHSVdk 100, 100, 95 98 5.0 ± 0.34 nt
 rVHSVdk-Gmi 100, 100 100 5.1* nt
 rVHSVdk-GNVLmi 100, 100, 100 100 4.64 ± 0.27 nt
 mock 0, 0, 0 0 na 0/9
  1. aWithin each experiment, all treatment groups were tested as triplicate subgroups of 20 juvenile rainbow trout, with the exception of mock challenge negative control treatments or wild type strain positive control treatment that were tested in some experiments as single or duplicate groups (as indicated in column 2) due to space limitations in the aquatic Biosafety Level 3 wetlab. Also, duplicate subgroups of 20 fish were used for the reciprocal G exchange chimeras in experiment 2 because this was a repeat test of this treatment group from experiment 1
  2. bMean day-to-death (MDD) for individual tanks was used to calculate the group average MDD and standard deviation (SD). *Asterisks denote MDD for groups that had mortality data from only 1 or 2 tanks, either due to tanks with no mortality or less than 3 tanks in the treatment. In these cases, no SD could be calculated. “na” means all tanks in the treatment group had no mortality
  3. cInfection status at 7 days post-challenge is shown for fish sampled from rVHSVmi-based viruses in experiments 1 and 2. For virus-positive fish, the number of fish in each group with viral loads greater than the challenge inoculum is shown in parentheses as an indication of the ability of the virus to replicate in vivo. The infectious viral loads determined by plaque assay are shown in Fig. 5