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Table 1 Oligonucleotides used for cloning, sequencing and mutagenesis of VHSV DK-3592B cDNA fragments

From: The glycoprotein, non-virion protein, and polymerase of viral hemorrhagic septicemia virus are not determinants of host-specific virulence in rainbow trout

Primers

Sequences

Nucleotide no.a

VHSV-DK genome primers

 VHSV 1F

GTATCATAAAAGATGATGAGT

1–21

 VHSV 1R

CAGCTTGAACTTCTTCATGGC

2007–1987

 VHSV 2F

AAGAAGACCGACAACATACTCT

1837–1858

 VHSV 2R

GACGAAACTTTGAGAGGAGAAA

3971–3950

 VHSV 3F

ATCTCATCACCAACATGGCTCAAA

3870–3893

 VHSV 3R

TTGTTCGCCTCTCCCCTAATTGT

5926–5888

 VHSV 4F

CCGCTATTGACTTGCTCAAATTG

5789–5812

 VHSV 4R

CTGATCCATGGTGGCTATGTGAT

8020–7998

 VHSV 5F

AGATGATTGTCTCCACCATGAA

7824–7845

 VHSV 5R

GAGATCCGCTCTCGTTCATCAA

10,005–9984

 VHSV 6F

GACAAGAAAGCTGGGAAGAGA

9765–9785

 VHSV 6R

GTATAGAAAATAATACATACCA

11,159–11,138

 VHSV 1MF

GGACAAGATGATCAAGTACATC

595–616

 VHSV 2MF

CCATTCTCTGCGAAGATCAACA

2435–2456

 VHSV 3MF

GATAAAGAAACATGGCGACCCA

4547–4568

 VSHV 4MF

GGATCATCAGGAAGAGACACCA

6392–6413

 VHSV 5MF

AGGCACACCACTTCACCATCCA

8412–8433

 VHSV-DK 5.3R

CCTTGGATTCTTGTTGAGTCA

5280–5260

Mutagenesis primers

 VHSV-DK PmlF

TTGTGTATACAACACGTGAGACCCACAATG

 

 VHSV-DK PmlR

CATTCCATTGTGGGTCTCACGTGTTGTATACACAA

 

 VHSV SacIIF

GTCTGAACACACCGCGGCGAATGACCACAATTCC

 

 VHSV SacIIR

GGAATTGTGGTCATTCGCCGCGGTGTGTTCAGA

 

 VHSV NheF

CCTCAGACAAGCTAGCACAAAAAAC

 

 VHSV NheR

GTTTTTTGTGCTAGCTTGTCTGAGG

 
  1. aThe positions where the primers bind (nucleotide numbers) are according to the published sequence of VHSV DK-3592B genome in the GenBank under the accession number KC778774 (Yusuff and Vakharia, 2013)