Fig. 5From: Noncovalent SUMO-interaction motifs in HIV integrase play important roles in SUMOylation, cofactor binding, and virus replicationHIV IN SIMs affect the nuclear localization of IN. The potential effects of IN SIMs on the nuclear localization of IN were evaluated in HeLa cells. a HeLa cells transfected with different GFP-INwt/mut (green) were analyzed for their subcellular localization by indirect fluorescence assay. The nuclei were stained with DAPI (blue). b 293 T cells were transfected with GFP-INwt or 3VI for 48 h. The subcellular distribution of INwt and 3VI was determined by the subcellular fractionation method. Ct, cytoplasmic extracts; Nu, nuclear extracts; Chr, chromatin bound extract. The ratio of nuclear portion (a combination of Nu and Chr from left panel) to the cytoplasmic fractionate of proteins was calculated and shown as a pie chart in the right panel. The nuclear localized portions of GFP-INwt and 3VI in the total protein extracts are approximately 80 and 40%, respectivelyBack to article page