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Fig. 5 | Virology Journal

Fig. 5

From: Noncovalent SUMO-interaction motifs in HIV integrase play important roles in SUMOylation, cofactor binding, and virus replication

Fig. 5

HIV IN SIMs affect the nuclear localization of IN. The potential effects of IN SIMs on the nuclear localization of IN were evaluated in HeLa cells. a HeLa cells transfected with different GFP-INwt/mut (green) were analyzed for their subcellular localization by indirect fluorescence assay. The nuclei were stained with DAPI (blue). b 293 T cells were transfected with GFP-INwt or 3VI for 48 h. The subcellular distribution of INwt and 3VI was determined by the subcellular fractionation method. Ct, cytoplasmic extracts; Nu, nuclear extracts; Chr, chromatin bound extract. The ratio of nuclear portion (a combination of Nu and Chr from left panel) to the cytoplasmic fractionate of proteins was calculated and shown as a pie chart in the right panel. The nuclear localized portions of GFP-INwt and 3VI in the total protein extracts are approximately 80 and 40%, respectively

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