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Fig. 1 | Virology Journal

Fig. 1

From: Noncovalent SUMO-interaction motifs in HIV integrase play important roles in SUMOylation, cofactor binding, and virus replication

Fig. 1

The three putative SIMs of IN and mutagenesis analysis. a Schematic of HIV-1 IN with its three putative SIMs and SUMO conjugation sites highlighted. IN contains three ψ-K-x-E SUMO conjugation sites (K46, K136 and K244) [7] and three putative SIMs. Four residues that constitute the hydrophobic core of each SIM are indicated (SIM1 72VILV75; SIM2 200IVDI203 and 257IKVV260). b The alignment of IN SIMs with other SIM-containing proteins, SUMO E3 protein RANBP2 and PIAS1 and PIASx. The SIM sequences are boxed with the hydrophobic amino acids highlighted in red. The acidic residues within 10 amino acids of the SIMs are shaded. c Three putative SIMs sequence alignment of different HIV-1 subtypes and HIV-2, SIVcpz integrase. d Mutagenesis analysis of IN SIMs. The point mutations introduced in the IN SIMs are shown in red. The IN SIMs were individually or simultaneously mutated to M1, M2, M3 or 3VI

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